The simultaneous determination of different posttranslational modifications of proteins – A comparison of approaches
Autoři | |
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Rok publikování | 2011 |
Druh | Konferenční abstrakty |
Fakulta / Pracoviště MU | |
Citace | DYČKA, Filip, Richard ČMELÍK a Janette BOBÁLOVÁ. The simultaneous determination of different posttranslational modifications of proteins – A comparison of approaches. In 29th Informal Meeting on Mass Spectrometry. 2011. ISBN 978-88-89884-19-5. |
Popis | This work reports enrichment techniques of the phosphopeptides and peptides containing cysteine sulfonic acid residue using IMAC tips containing iron ions and titanium dioxide chromatography. The methods were optimized using tryptic peptides of alpha-casein and albumin. After enrichment procedure the modified peptides were analyzed by MALDI-TOF. |
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